"Huijsen arrived with symptoms of muscular fatigue at the Spain national team camp on Monday night," said the Spanish football federation in a statement.

"On Tuesday he did not train... and on Wednesday he had a medical test confirming a muscular injury, which Real Madrid have been informed of."

Real Madrid said Huijsen has suffered a "calf muscle injury in his left leg", without specifying an expected recovery date.

Spanish media reported he is likely to return in 10-12 days, putting in doubt Huijsen being fit for Madrid's La Liga game at Getafe on October 19.

The 20-year-old centre-back joined Real from Premier League club Bournemouth this summer and made his Spain debut in March in the Nations League.

Laporte, who recently rejoined Athletic Bilbao, was a key part of the Spain squad that won Euro 2024.

Barcelona winger Lamine Yamal and Manchester City midfielder Rodri Hernandez had already withdrawn from the squad for the games against Georgia and Bulgaria on Saturday and October 14 respectively.

Spain top Group E after beating Bulgaria and Turkey in their opening qualifiers for next summer's tournament in the United States, Mexico and Canada.